Journal Article

Production and use of an antiserum to grapevine virus B capsid protein purified from SDS-polyacrylamide gels

Goszczynski, D. E.,Kasdorf, G. G. F.,Pietersen, G.

Year1997

JournalVitis

Volume36

Total pages4

Keywordsantibodies, detection, ELISA, GRAPEVINE, grapevine B trichovirus, grapevine A trichovirus, grapevine virus B, plant diseases, plant pathogens, detection, immunodiagnosis, immune serum, ELISA, cross reaction, coat proteins, grapes, fruit crops, plant pathology, plant viruses, Vitis, production, protein, VIRUS, viruses, plant pathogens, pathogens, Vitidaceae, Rhamnales, dicotyledons, angiosperms, Spermatophyta, plants

Abstract

Antiserum to electrophoretically-separated capsid protein of grapevine virus B [grapevine B trichovirus] (GVB) was produced. After easy and effective elimination of antibodies cross-reactive with grapevine virus A [grapevine A trichovirus] (GVA), the antiserum was successfully used in ELISA for the detection of GVB in grapevines