Journal Article
Molecular characterisation of a strain of peanut stripe potyvirus from groundnut germplasm imported into South Africa
Year1999
JournalAfrican Plant Protection
Volume5
Total pages1
KeywordsAfrica, Africa, South Africa, USA, analysis, Arachis hypogaea, plant viruses, Arachis, Papilionoideae, Fabaceae, Fabales, dicotyledons, angiosperms, Spermatophyta, plants, viruses, plant pathogens, pathogens, Southern Africa, Africa South of Sahara, Africa, Developing Countries, Threshold Countries, Anglophone Africa, Commonwealth, coat, COAT PROTEIN, COAT PROTEIN GENE, electron microscopy, GENE, germplasm, HOST, host range
Abstract
Peanut stripe virus (PStV) was identified in a groundnut plant grown from seed imported into South Africa. Host range, serology and electron microscopy studies indicated that the infective agent was an isolate of PStV. This was confirmed by sequencing the coat protein gene and a portion of the 3' untranslated region of the virus and comparing it with known strains of PStV. The local strain (PStV-95/399) was related to, but distinct from, strains of PStV reported from the USA. PStV-95/399 and the USA strains probably had a common ancestor, but the 95/399 sequence appeared to have diverged to a greater extent from this ancestral sequence. Phylogenetic analysis indicated that 95/399 probably originated in the USA