Journal Article
Detection of two strains of grapevine leafroll-associated virus 2
Year1996
JournalVitis
Volume35
Total pages3
KeywordsAfrica, CAPSID PROTEINS, CLOSTEROVIRUS, detection, development, GRAPEVINE, grapevine leafroll-associated closterovirus, Closterovirus, lesions, mechanical transmission, plant diseases, plant pathogens, serology, grapes, strains, fruit crops, plant pathology, plant viruses, viruses, Vitis, protein, proteins, South Africa, South Africa, strain, strains, symptoms, transmission, VIRUS, viruses, plant pathogens, pathogens, Vitidaceae, Rhamnales, dicotyledons, angios
Abstract
Two strains of grapevine leafroll-associated closterovirus 2 (GLRaV-2) were obtained by mechanical transmission from grape to <i>Nicotiana benthamiana</i> in South Africa. The strains, designated 94/970 and 93/955, consistently differed with regard to the development of symptoms. The first induced chlorotic and occasional white-necrotic local lesions, while the second induced chlorotic followed by metallic-opalescent, solid necrotic local lesions. The strains were indistinguishable with regard to the MW of their capsid proteins or serologically. A difference in the pattern of minor dsRNA bands was consistently observed