Berberis lycium: The newly discovered aecial host of Puccinia striiformoides

Berberis lycium is reported for the first time as the alternate (aecial) host for Puccinia striiformoides from collections in Pakistan. This finding is confirmed by phylogenetic analyses of internal transcribed spacer (ITS) rDNA sequence data, which place our sequences of aecial P. striiformoides on B. lycium in a well-supported clade with GenBank sequences from uredinial P. striiformoides on Dactylis glomerata . Aecia of Puccinia graminis , P. graminis f.sp. tritici , and P. brachypodii that were also collected on B. lycium from Pakistan provide additional evidence of multiple rust species occurring on secondary host plant species.